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primary mouse skeletal muscle satellite cells  (iXCells Biotechnologies)


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    iXCells Biotechnologies primary mouse skeletal muscle satellite cells
    Fig. 2. Nucleolar re-organization in <t>satellite</t> <t>cells</t> from <t>mouse</t> hindlimb <t>muscle.</t> Mouse <t>skeletal</t> myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.
    Primary Mouse Skeletal Muscle Satellite Cells, supplied by iXCells Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+skeletal+muscle+satellite+cells/Mouse+Skeletal+Muscle+Myoblasts/pm36727534-200-0-9
    Average 94 stars, based on 5 article reviews
    primary mouse skeletal muscle satellite cells - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Re-organization of nucleolar architecture in myogenic differentiation."

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.

    Journal: Journal of cell science

    doi: 10.1242/jcs.260496

    Fig. 2. Nucleolar re-organization in satellite cells from mouse hindlimb muscle. Mouse skeletal myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.
    Figure Legend Snippet: Fig. 2. Nucleolar re-organization in satellite cells from mouse hindlimb muscle. Mouse skeletal myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.

    Techniques Used: Derivative Assay, Muscles, Immunofluorescence, Staining, Fluorescence, Microscopy, Generated, Software

    Related Articles

    Derivative Assay:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Muscles:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Immunofluorescence:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Staining:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Fluorescence:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Microscopy:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Generated:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.

    Software:

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.
    Article Snippet: Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.Myotube formation was induced by transferring C2C12 cells to differentiation medium (DM; DMEM with 2% FBS) upon reaching 80– 90% confluency.. Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).. These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.These cells are derived from mouse skeletal muscle satellite cells isolated from mouse hindlimb gastrocnemius and tibialis anterior muscles.



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    primary mouse skeletal muscle satellite cells  (iXCells Biotechnologies)
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    iXCells Biotechnologies primary mouse skeletal muscle satellite cells
    Fig. 2. Nucleolar re-organization in <t>satellite</t> <t>cells</t> from <t>mouse</t> hindlimb <t>muscle.</t> Mouse <t>skeletal</t> myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.
    Primary Mouse Skeletal Muscle Satellite Cells, supplied by iXCells Biotechnologies, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+mouse+skeletal+muscle+satellite+cells/Mouse+Skeletal+Muscle+Myoblasts/pm36727534-200-0-9
    Average 94 stars, based on 1 article reviews
    primary mouse skeletal muscle satellite cells - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 2. Nucleolar re-organization in satellite cells from mouse hindlimb muscle. Mouse skeletal myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.

    Journal: Journal of cell science

    Article Title: Re-organization of nucleolar architecture in myogenic differentiation.

    doi: 10.1242/jcs.260496

    Figure Lengend Snippet: Fig. 2. Nucleolar re-organization in satellite cells from mouse hindlimb muscle. Mouse skeletal myoblasts (satellite cells) derived from mouse hindlimb gastrocnemius and tibialis anterior muscles were seeded onto glass-bottom dishes with GM (20% FBS). Upon growth to high density, the cells were transferred to DM (2% FBS) and maintained for 4 days. The cells were fixed and subjected to immunofluorescence analysis for nucleolin (red) and MyHC (green) (A), or fibrillarin (red) and F-actin (green) (B). The nuclei were stained by Hoechst 33342 (blue). Z-stack images (240 nm intervals) were obtained by confocal fluorescence microscopy, and orthogonal projection images were generated using Zen software (Zeiss). The size of each nucleolus was measured using ImageJ software. Fold increases (area) were calculated relative to the average size of the nuclei in the MBs and graphed, error bars represent s.d. Numbers on the bar graph indicate the number of nucleolar measurements. NS, not significant (P>0.05); **P<0.001; ***P<0.0001 (one-way ANOVA with Tukey HSD post test). MB, in myoblasts; MT, in myotubes; RC, in reserve cells.

    Article Snippet: Primary mouse skeletal muscle satellite cells were obtained from iXCells Biotechnologies (10MU-033).

    Techniques: Derivative Assay, Muscles, Immunofluorescence, Staining, Fluorescence, Microscopy, Generated, Software